High throughput single-cell detection of multiplex CRISPR-edited gene modifications

High throughput single-cell detection of multiplex CRISPR-edited gene modifications

Blog Article

Abstract CRISPR-Cas9 gene editing has transformed our ability to rapidly interrogate the functional impact of somatic mutations in human cancers.Droplet-based technology enables the analysis of Cas9-introduced gene edits in thousands Ski de fond - Femme - Bottes - Skate of single cells.Using Bedroom Package this technology, we analyze Ba/F3 cells engineered to express single or multiplexed loss-of-function mutations recurrent in chronic lymphocytic leukemia.Our approach reliably quantifies mutational co-occurrences, zygosity status, and the occurrence of Cas9 edits at single-cell resolution.